IJFAF | Determination of the Prevalence of Bluetongue Disease in Goats in Siirt Province by the ELISA Method

Indexing and Abstracting

Determination of the Prevalence of Bluetongue Disease in Goats in Siirt Province by the ELISA Method

Ozgur Yasar CELIK , Tekin ŞAHİN , Gülşah AKGÜL

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DOI: 10.22161/ijfaf.2.6.1

Journal : International Journal Of Forest, Animal And Fisheries Research(IJFAF)

Abstract:

Bluetongue disease is a disease caused by the bluetongue virus in the Orbivirus genus of the Reoviridae family. The disease can be transmitted by ticks, sperm and vertically but is mainly spread by the Culicoides type flies. While the disease is naturally observed in cattle and sheep, it is more rarely observed in goats. This study was carried out to determine the presence of bluetongue infection in goats raised in Siirt province serologically and to obtain information about its prevalence in the region. The material of the study consisted of a total of 372 goats over one year of age raised in Siirt province. For laboratory analyses, 5 ml blood samples were taken from the jugular vein of goats to tubes without anticoagulant. The samples were centrifuged at 3000 rpm for 10 minutes, and the sera were transferred to Eppendorf tubes and stored at -20 Â°C until analysis. The serum samples were analyzed by the ELISA (Thermo Scientific Multiskan Go) method using the commercial test kit (Bluetongue Virus VP7 Ab Test Kit, IDEXX). The results were evaluated as positive and negative. Of the 372 goats constituting the material of the study, 252 (67.74%) were seropositive and 120 (32.26%) were seronegative. As a result, in addition to measures for quarantine and vector control in the fight against the disease, it was concluded that vaccines specific to virus serotypes should be used and comprehensive and planned studies should be carried out in relation to the disease in the region and the country.

Keywords:

Bluetongue, ELISA, Goat, Siirt, Turkey

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